http://barrick.mmg.msu.edu/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2009 by contributing authors Jeffrey Barrick [webmaster@barricklab.org] The contributing authors of TWiki TWiki http://barrick.mmg.msu.edu/twiki/bin/view/Lab http://barrick.mmg.msu.edu/twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif http://barrick.mmg.msu.edu/twiki/bin/view/Lab/PublicationList Publications Barrick JEPublication Network Graph by 26. Barrick, J.E. , Yu, D.S., Yoon, S.H., Jeong, H, Oh, T.K., Schneider, D., Lenski, R.E., and Kim, J.F. 2009 ... (last changed by JeffreyBarrick) 2009-10-24T00:25:52Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ContactInformation Contact Information Jeffrey E. Barrick, Ph.D. email: jbarrick at msu dot edu Research Associate Laboratory of Richard Lenski Microbiology and Molecular Genetics ... (last changed by JeffreyBarrick) 2009-10-07T12:47:32Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProceduresLabelTemplates Label Templates Templates for printing labels... (last changed by JeffreyBarrick) 2009-09-29T15:28:51Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProtocolList Protocols and Laboratory Reference Organization Label Templates Sequence Analysis Primer Design PCR Gene and Genomic Sequencing Bacterial ... (last changed by JeffreyBarrick) 2009-09-29T15:24:49Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProceduresASKAEvolutionExperiment ASKA Collection Evolution Experiment Starting Lines 1 Revive each strain by inoculating a scrape of frozen culture from a well of a microplate into 3 ml of 0.1x ... (last changed by JeffreyBarrick) 2009-09-23T21:15:51Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/WebLeftBar Home Contact Research Publications Supplements Tools Protocols Reference ' '' " then "" else ""}% (last changed by JeffreyBarrick) 2009-09-23T21:03:42Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ResearchInterests Research Interests /collage.png Overview I am broadly interested in understanding evolution as a creative force. My laboratory will use experiments with populations ... (last changed by JeffreyBarrick) 2009-09-23T21:02:21Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProjectsManBCpsGDeletion manB cpsG deletion 1 Amplify negative and positive selection cassette encoding sacB and cat from pKO3 adding genome specific regions to each end. Try two different ... (last changed by JeffreyBarrick) 2009-08-22T19:10:34Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProjectsCloningStrainConstruction Cloning and Strain Construction Projects Strain library evolution experiments KanR and empty PCA24N plasmids as ASKA controls Mal version of AG1 to follow ... (last changed by JeffreyBarrick) 2009-08-22T18:41:29Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProceduresPulsedFieldGelElectrophoresisMapping Pulsed Field Gel Electrophoresis Mapping Purpose: To validate mutations predicted from whole genome re sequencing and possibly discover rearrangements through repetitive ... (last changed by JeffreyBarrick) 2009-08-17T22:40:14Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProjectsInfB nop infB strain construction 1 REL606 with infB1 allele from JEB154. 1 JEB154 with ancestral REL606 allele. primer name sequence RL1041 ... (last changed by JeffreyBarrick) 2009-08-17T18:03:43Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ReferenceList Reference Information Lenski Long Term E. coli Lines Plasmids Sequence of REL606 Installation Workarounds (last changed by JeffreyBarrick) 2009-08-16T22:09:09Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/SupplementRiboswitchPrediction Predicting Riboswitch Regulation on a Genomic Scale This page describes how to install a set of Perl scripts designed to identify members of known classes in genomic ... (last changed by JeffreyBarrick) 2009-08-15T13:47:39Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/WebPreferences nop Lab Web Preferences The following settings are web preferences of the Lab web. These preferences overwrite the site level preferences in . and ... (last changed by JeffreyBarrick) 2009-08-14T17:45:26Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/ProceduresGenomicDNAResequencing Genomic DNA Preparation for Whole Genome Re sequencing of E. coli Current RTSF Instrument Information 454 Pyrosequencing (GSFlex machine) Read length is 100 ... (last changed by JeffreyBarrick) 2009-08-12T20:22:52Z JeffreyBarrick http://barrick.mmg.msu.edu/twiki/bin/view/Lab/InstallationWorkarounds Math::GSL 0.20 On MacBook Pro: Architecture set incorrectly to arch ppc when compiling some files due to Perl Config module. Can edit inc/GSLBuilder.pm to fix. On ... (last changed by JeffreyBarrick) 2009-08-10T17:49:54Z JeffreyBarrick