Cloning and Strain Construction Projects

Strain library evolution experiments

• Kan^R and empty PCA24N plasmids as ASKA controls
• Mal^- version of AG1 to follow experiment
• Cam^R version of BW25113 to follow experiment

• Isogenic infB1 replacements and infB allele on fitness
• Restore thyA in evolved ΔthyA strains.
• Create manB-cpsG deletion

• Move Kishony cfp/yfp into REL606 background