Liquid Media

LB

Synonyms: TBYE

10 g Tryptone
5 g Yeast Extract
5 g NaCl

Add dH2O to 1 liter. Autoclave.

Luria-Bertani

Note: This is different from LB media!

10 g Tryptone
5 g Yeast Extract
10 g NaCl

Add dH2O to 1 liter. Autoclave.

DM: Davis Minimal

7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 (H2O)3 MW 228.17
5 g Potassium Phosphate (monobasic) H2KPO4 MW 136.07
1 g Ammonium Sulfate (NH4)2 SO4 MW 132.08
0.5 g Sodium Citrate (trisodium, dihydrate) Na3C6H5O7 (H2O)2 MW 294.10
Add dH2O to 1 liter. Autoclave. Then add
1 ml 10% Magnesium Sulfate MgSO4 MW 120.37
1 ml 0.2% Thiamin (vitamin B1)  

Final nutrient concentrations:

67.4 mM Phosphate (PO43-)
8.38 mM Sulfate (SO42-)
15.1 mM Ammonium (NH4)
5.1 mM Sodium (Na+)
98.1 mM Potassium (K+)
0.83 mM Magnesium (MGgsup>2+)
1.7 mM Citrate
139 µM Glucose (in DM25)

NZY+ Broth

10 g NZ Amine (casein hydrolysate)
5 g Yeast Extract
5 g NaCl

Add dH2O to 950 ml. Adjust pH to 7.5 using NaOH (Requires ~350 µl of 10 N). Autoclave. Then add.

12.5 ml 1 M MgCl2
12.5 ml / 15.0 ml 1 M / 10% (w/v) MgSO4 FW = 120.3 g/mol
40 ml 10% (w/v) glucose

Solid Media

DM: Davis Minimal

7 g Potassium Phosphate (dibasic, trihydrate) HK2PO4 H2O
5 g Potassium Phosphate (monobasic) H2KPO4
1 g Ammonium Sulfate (NH4)2 SO4

Add dH2O to 0.5 liter. Autoclave.

16 g / 10 g Agar or Agarose
1 ml 5% Antifoam

Add dH2O to 0.5 liter. Autoclave.

Combine and add

1 ml 10% Magnesium Sulfate MgSO4
1 ml 0.2% Thiamin (vitamin B1)

Important! Do not combine agar and phosphate solutions before autoclaving.

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Topic revision: r4 - 09 Sep 2007 - 16:23:26 - JeffreyBarrick
 
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