Genomic DNA Preparation for Whole-Genome Re-sequencing of E. coli

Current RTSF Instrument Information

454 Pyrosequencing (GSFlex machine)

• Read length is 100-200 bases.
• Paired end sample prep kit available.
• Two kits with 6-8 primer tags available. Possibility of bar coding up to 16 different samples per area of plate.

Illumina Genome Analyzer 2G

• 1-5 µg of gDNA needed per sample.
• Read length is 36 bases or 72 bases if two sequencing kits are used.
• Paired end reads available. Two different protocols for insert sized of 100-200 bp or 3-5 kbp.
• Eight wells on slides. One reserved for control or previously sequenced sample.
• Submit 50 µl of 100 ng/µl sample in 1 x TE to RTSF.